Molecular Mechanism of lncRNA NR2F2-AS1 Regulating CTGF in Helicobacter pylori-Induced Gastric Epithelial Cell Injury

Abstract

Objective: To investigate the role of lncRNA NR2F2-AS1 and its regulation of CTGF in Helicobacter pylori-induced gastric epithelial injury.. Methods: GES-1 cells were infected with H. pylori (MOIs 50–200). Cell viability, proliferation, and migration were assessed alongside the expression of NR2F2-AS1, CTGF, and pro-inflammatory cytokines (IL-6, IL-8). The regulatory link was verified using stable NR2F2-AS1 overexpression. Results: H. pylori infection significantly upregulated NR2F2-AS1 and CTGF, leading to dose-dependent decreases in cell viability, suppressed proliferation, and impaired migration (p < 0.01). NR2F2-AS1 overexpression further boosted CTGF levels and exacerbated inflammatory responses. FISH localized NR2F2-AS1 primarily to the cytoplasm, suggesting post-transcriptional regulation. Conclusion: NR2F2-AS1 promotes H. pylori-induced injury by positively regulating CTGF. This axis represents a potential therapeutic target for mitigating gastric mucosal damage.